Department of Physics, Chemistry and Biology (IFM)

Results from semi quantitative PCR with genes from the Notch pathway in HT29-APC and HT29-βgal. Diagrams are showing the intensity signal from HT29APC/HT29-βgal at different time points. There is a clear trend of downregulation, lowering of signal in 24h-6h samples for all genes except GAPDH. Experiments were repeated twice. Western blot against APC was preformed and APC was upregulated in APC 24-6h (data not shown). Click to enlarge graph.

Results from semi quantitative PCR with genes from the Notch pathway in HT29 cells transfected with siRNA against β-catenin. Diagrams are showing the intensity signal from siRNA transfected/siRNA transfected 0h in different time points. There is a clear trend of downregulation, lowering of signal in 96h-24h samples. Experiments were repeated twice. Western blot against β-catenin was preformed and β-catenin was downregulated in all timepoints execept 0h. Click to enlarge graph.

Results from DAPT,DMSO and Mock treatment of HT29 cells for 48h. Diagrams are showing intensity signal from the different treatments/Mock 2. Experiments were repeated twice.   
Western blot against HES1 was preformed and HES1 was downegulated in DAPT treated cells (data not shown). Click to enlarge graph.