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Quantitiative real-time PCR

An upregulation was seen in of the genes PpLTPg6 during UV-B radiation stress, in PpLTPg3, PpLTPg8 and PpLTPg9 during cold stress, and in PpLTPg2, PpLTPg4 and PpLTPg6 during dehydration stress.

The graph below shows all the significantlly upregulated genes.

An upregulation of the PpLTPg genes during various abiotic stresses. The values are normalized and compared to the control sample. The asterix (*) represent significance of p<0.05, calculated with permutation test.

 

Phylogenetic tree

Additionally, a phylogenetic tree was constructed to show the relationship between the upregulated genes (see below).

The green lines indicate the type G genes, while the red lines indicate the type D gene. PpLTPg2, PpLTPg3, PpLTPg4, PpLTPg6, PpLTPg8 and PpLTPg9 all seem to share a common origin, while PpLTPg1, PpLTPg5, PpLTPg7 and PpLTPg10 seem to share a different common origin. Interestingly, the genes PpLTPg2, PpLTPg3, PpLTPg4, PpLTPg6, PpLTPg8 and PpLTPg9, who all were upregulated in some of the abiotic stresses, are all found in the same branch 

 

A phylogenetic tree of the PpLTPg genes. The upregulated genes during the various stresses are indicated. The numbers on the axis represents the bootstrap values.


 


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Last updated: 06/06/11