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Sample

The fresh pig blood samples used for this study were obtained from a slaughter house in Erlangen ( Bavaria , Germany ). The pigs were cut at the throat and the blood was collected directly into glass bottles which already contained a 0.1 M sodium citrate solution (Alfa Aesar, Karlsruhe , Germany ) to prevent coagulation. The volume of the sodium citrate solution and the blood was in the ratio of 1:10 (50 ml of sodium citrate for 500 ml of blood). The samples were then immediately subjected to a SAFE (see below) without any further delay. A total of four blood samples from four different pigs were obtained for the identification of the odor active compounds in the pig blood

Reagents

Dichloromethane (DCM) was purchased from Acros organics ( Nidderau , Germany ). Water used to prepare the sodium citrate solution was purified in a Micropure system from TKA ( Niederelbert , Germany ). An alkane solution    (C6-C34) in DCM was used to calculate the retention index values of the analytes in the blood samples. The following reference compounds were used for the identification of the blood volatiles: 2,3 butadione (Fluka, Neu-Ulm, Germany), 2-methyl nonanoic acid (Alfa Aesar, Karlsruhe, Germany), vanillin,   δ -octalactone (ABCR, Karlsruhe, Germany), trans-4,5-epoxy-(E)-dec-2-enal, (Z)-octa-1,5-dien-3-one (Aromalab, Freising, Germany), acetic acid, Z-4-heptenal, octanal, 3-(methylthio)-propanal (methional), 4-methyl phenol, 1-octen-3-one, hexanalphenyl aceticacid, δ -nonalactone, 4-methyl nonanoic acid, androstenone ( 5 α -androst-16-en-3-one) (Aldrich, Steinheim, Germany), androstadienone ( androsta-4,16,-dien-3-one ) (Steroids Ltd, Illinois, USA)


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Last updated: 05/19/12