For the behaviours I found one principal component that differed between the two groups containing behaviours that indicate anxiety and stress. Which is in line with previous research on this type of behaviour studies. 

For the hair cortisol I found no significant dofferences. This might be dure to the fact that I had mixed and small groups with different agees, sex and breeds. Which are all important factors that contribute to how stress hormones will look in the hair. 

Dogs with separation anxiety had less resting activity during separation compared to control dogs that had more resting activity. Dogs with separation anxiety also had more medium activity during separation compared to control dogs. 

Dogs with separation anxiety had no differences in rest, low and medium activity during separation compared to owner present. However there was a significant difference in high activity that was performed more when owner present compared to separation. The control dogs had significant differences in all four activity levels. With more rest activity and less low, medium and hig activity during separation compared to owner present. 

What does this mean

• Control dogs are passive or relaxed
• Dogs with separation anxiety are restless 

The correlation analysis to  separation anxiety showed correlations with

• separation related problems
• attachment
• Anxiety related behaviours (principal component 1)

This indicates that dog owner do know if their dogs suffer from separation anxiety or not.

• There is a difference in behaviour between dogs with separation anxiety and control dogs
• No differences between the two groups in regards of hair cortisol
• Activity monitoring can be used to investigate separation anxiety
• C-BARQ can be used to phenotype separation anxiety in Swedish dogs