I found that Prdm2 knockdown did not affect innate anxiety levels. Indeed, the two experimental groups did not show any significant difference either in the relative time spent on the open arms of the elevated plus maze (Fig.4a) or in the locomotory activity in the open field test (Fig.4b). 

However, Prdm2 knockdown had a significant effect in the cued fear conditioning paradigm. Specifically, Prdm2 knockdown rats showed higher freezing behaviour than controls (Fig,5).

Despite the fact that there were no significant differences among the groups, both witnesses and stressed subjects showed a tendency for higher anxiety-like behaviour than controls. Indeed, witnesses and stressed subjects spent less time on the open arms of elevated plus maze than controls (Fig.6a). Moreover, witnesses and stressed subjects spent less time in the centre of an open field than controls (Fig.6b).