IFN-gamma was an important factor in producing ANoA in mercury induced autoimmunity. CD4⁺CD25⁺ showed no role in the production of ANoA.  From all the result ELISA obtained, we can conclude that in our experiment, the T regulatory cells failed to suppress Mercury induced Autoimmunity. As the expected result was that, CD4⁺CD25⁺ can supress HgIA, further studies should be done in order to understand the mechanism of ANoA production and the mechanism of CD4⁺CD25⁺ cells.