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Protein identification

Three novel SR-proteins in P. patens were discovered PpSR40, PpSR36 and PpSR39. The novel proteins all have two RRM (RNA recognition motif) domain and SR dipeptide repeats towards the c-terminal of the proteins.

Phylogenetic analysis

The phylogenetic analysis suggests that the proteins are the product of two gene duplications and subsequent sequence divergence.

 

Tissue-specific gene expression analysis

The expression of the PpSR40 and PpSR36 genes were upregulated in protonema tissue whereas the expression of PpSR39 was similar in the two tissues

Tissue-specific gene expression analysis by RT-PCR show significant differential expression of PpSR40 and PpSR36 genes in protonema (Data is presented as means ± SD fold-change to gametophyte, *: p=0.05, one-way ANOVA, N=2, Tukey and Fisher method post-hoc analysis.

Overexpression of PpSR40 in P. patens

The six PpSR40 overexpression lines vary in the degree of overexpression of PpSR40-transcript. Overexpression did not significantly alter the splicing patterns of PpSR40, PpSR36 and PpSR39.

Gene expression analysis of six PpSR40 overexpression lines. Expression of PpSR40 vary between the lines but all show higher expression levels than wild-type (Data is presented in fold-change to wild-type control).


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Last updated: 05/15/11