The regulation of egg temperature requires the transfer of heat from the brood patch. Thus, the broodpatch needs (1) the presence of thermo receptors and (2) an appropriate vasomotor response. During the incubation an exact detection of the egg’s temperature is essential. In this study we tried to detect the presence and regulation of thermoTRP channels (thermo Transient Receptor Potential channels) (TRPV1, TRPV3, TRPV4, TRPM8 and TRPA1) expressions during egg incubation. Six incubating Jungle fowl hens, and five non incubating jungle fowl hens and one jungle fowl cock were used as main samples and controls. Total RNA was extracted from liver, kidney, heart, blood, WBC (White Blood Cell), DRG (Dorsal Root Ganglion) and skin. The samples from the skin were taken from the brood patch and interscapular region. PCR investigation showed different thermoTRP channels are expressed in different tissues but we did not find TRPV4 mRNA in any tissue. TRPV1, V3 and M8 mRNA was detected in the skin of brood patch. However, V1 and V3 expression in the broodpatch skin did not differ between broody and non broody hens. In conclusion, although considerable morphological changes in the skin of brood patch could be seen the tested thermoTRP channel’s expression did not show significant change