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The qPCR results analyzed by the REST method showed that TRPV3 was not significantly different in the skin of brood patch for broody hens compared to non broody hens.

TRPV1 expression was very low and the results showed that the expression did not change significantly either. TRPM8 primers did not work with any of the Master mixes we tried for real time quantitative PCR.

The brood patch skin changed markedly during incubation. The epidermal thickness increased significantly in the skin of brood patch in the broody compared to non broody hens (p=0.001). The epidermis was also thicker in the skin samples of brood patch compared to the samples from inter scapular region in the incubating hens (p=0.004).

The frequency of AVAs (arteriovenous anastomosis) increased significantly in the brood patch samples of broody jungle fowls compared to interscapular skin (p=0.012) and this did not occur in non broody birds. There was no difference in the AVA frequency between non broody and broody hens . 
 
Furthermore, the AVA area was larger not only in the brood patch of incubating jungle fowls in comparison with non incubating hens (p=0.02), but also in the brood patch versus inter scapular region (p=0.044).


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Last updated: 05/18/09