Department of Physics, Chemistry and Biology (IFM)

After immobilization step, the chip is tested for the antibody activity. Each channel which was immobilized with antibody of one kind was checked if there was any binding with the antigen of the same kind. The test was considered to be positive when there is specific binding between bacteria and the respective bacteria. Anti-guinea pig IgG was used as negative control while Anti-mouse IgG and Anti-human IgG were used as positive controls. The chip was also tested for different bacteria other than the bacteria against which the antibody was prepared and immobilized in their respective channels. The tables below show the response obtained with different bacteria and Fab specific antibodies as controls in each channel of the chip during purity test of the chip.