Department of Physics, Chemistry and Biology (IFM)

Ulcer secretions from breast cancer patients were run to detect the bacteria in each of the samples. Biacore response signals were obtained for each channel and specific binding of pathogenic bacteira present in the sample to the antibody immobilized on the channel was considered positive. Regeneration buffer was used for wash between the sample run. All samples were diluted in PBS in the ratio of 1:1 to enable detection.

The results from the sample run using ulcer secretions from patients with chronic leg ulcers (k1-k11), ulcer secretions from patients with acute ulcer post- breast cancer operation (1a- 12a), and ulcer secretion from skin biopsy of healthy volunteers (h1-h10).