Further testing of the candidate genes was conducted in a second population, which was a backcross between an F1 from a RJF x WL and a RJF parental.

• No correlation between colour and relative gene expression was found for the second most expressed candidate gene (top left panel).
• A strong positive correlation was found between the most highly expressed candidate gene and colouration (top right panel).
• Expression of the same gene was tested in the same individuals ten days later, and a similar positive correlation was found once more (bottom left panel), indicating that the results are  replicable.
• Expression of this candidate gene was also found to have a strong positive correlation with colouration in skin tissue which can bee seen in the bottom right panel.