My findings suggest that aversive stimuli early in life such as; social isolation, can lead to short term effects that were observed in body weight as well as long term effects that were represented in decrease in elevated levels of Il-6 as an indicator of disorders in the immune-system response. Environmental enrichment was shown to have positive effect on the immune system in bird. Moreover, the present study pinpoint the possibility of combining two methods (GBS,MeDIP) for detecting DNA methylation followed by sequencing. Therefore, we emphasis the relevance of studying the early life stress effect on epigenetic, immune system-response and behaviours. Moreover, studying the transgenerational effects of these aversive experiences as it is relevant on evolution levels.