When comparing estradiol levels between control and hatchery females, there was a tendency that hatchery females have higher levels of estradiol than control females (χ²=3.6674, p=0.06). There was also a significant interaction between treatment group and sample (χ²=4.0231, p=0.04). After conducting a post hoc Tukey test, there was no significant difference between control and hatchery female for the sample taken at 15 weeks of age (t=-0.052, p=0.9). However there was a significant difference between control and hatchery for sample 2 taken at 19 weeks of age (t=-2.772, p=0.04).

The effect of home pen was also analysed, there was no significant differences between pens (χ²=5.6142, p=0.1) and no interaction between pen and estradiol sample (χ²=6.2133, p=0.1). The post hoc analysis of the effect of pen on estradiol showed that there were no significant differences when comparing the results for the 15 weeks samples between pens (p>0.05). However when comparing pen differences for the 19 weeks sample there was a significant difference between pen 1 and 4 (t=-3.258, p=0.05). There were no other significant differences between the other pens when comparing the results for sample 2 (p>0.05).

When comparing testosterone levels between control and hatchery males, there were no significant differences between the testosterone levels of control and hatchery males (χ²=0.0001, p=0.9). After conducting a post hoc Tukey test, there was no significant difference between control and hatchery males for the sample taken at 15 weeks (t=-0.620, p=0.9) or at 19 weeks (t=0.635, p=0.9).

The effect of home pen was also analysed, there was a significant difference in testosterone between pens (χ²=5.6142, p=0.02) and the interaction between pens and testosterone samples (χ²=10.4755, p=0.01). The post hoc analysis of the effect of pen on testosterone showed that there were no significant differences when comparing the results for the sample taken at 15 weeks between pens (p>0.05). However when comparing pen differences for the sample taken at 19 weeks there was a significant difference between pen 2 and 3 (t=3.491, p=0.03), and pen 2 and 4 (t=-3.547, p=0.02). There were no other significant differences between the other pens (p>0.05).